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yloB [2019-01-04 10:24:56]
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yloB [2019-01-04 10:24:56]

ATP-driven Ca2+ pump, transports calcium ions from the mother cell to the forespore
locus
BSU15650
pI
8.71
mw
97.10 kDa
protein length
890 aa Sequence Blast
gene length
2670 bp Sequence Blast
function
accumulation of calcium in the spore
product
ATP-driven Ca2+ pump
essential
no
ec
3.6.3.8
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.4|Metal ion homeostasis/ Other]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,637,965 → 1,640,637

    Phenotypes of a mutant

  • forms unstable spores [pubmed|30602489]
  • delayed spore germination [pubmed|30602489]

    • The protein

    Catalyzed reaction/ biological activity

  • transport of calcium ions from the mother cell to the forespore [pubmed|30602489]
  • there is no indication for an implication of [protein|7821A09DADC6EAF589A882F05CC34BE900E06F2A|YloB] in Mg2+ uptake (based on mutant phenotypes, failure to complement mutants defective in Mg2+ uptake) [Pubmed|24415722]

    • Protein family

  • Type IIA subfamily (according to Swiss-Prot)

    • Structure

  • [PDB|4YCM] (the calcium pump with bound marine macrolide BLS from O. cuniculus, 35% identity) [pubmed|25957767]

    • [SW|Localization]

  • cell membrane [pubmed|30602489]

    • Expression and Regulation

    Operons

    genes
    [gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]-[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]

    description

  • [pubmed|22383849]

    • regulation

  • expressed during sporulation in the mother cell [pubmed|12161109]
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    Biological materials

    Mutant

  • MGNA-B139 (yloB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1138 NBRP B. subtilis, Japan]
  • BKE15650 (Δ[gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCGCTCGTCCACTCCC, downstream forward: _UP4_TAAATTCATATGATATAATC
  • BKK15650 (Δ[gene|7821A09DADC6EAF589A882F05CC34BE900E06F2A|yloB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCGCTCGTCCACTCCC, downstream forward: _UP4_TAAATTCATATGATATAATC

    • References

  • 24415722,28951477,25957767,30602489